Fluorescence Spectrophotometer
Lifetime fluorescence spectroscopy investigates the change in fluorescence over time of a sample when irradiated with UV, visible, or near-IR light. It involves a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and indicates them to emit light; typically, but not necessarily, visible light. A complementary technique is absorption spectroscopy.
JASCO Spectrofluorometer Model FP8300
Specification of the instrument:
| Make | JASCO SPECTROFLUOROMETER |
| Model | FP8300 |
| Spectral range | Zero order, 200-750 nm |
| Band width | 1, 2.5, 5, 10, 20 nm |
| Wavelength Accuracy | ± 1.5 nm |
In the special case of single molecule fluorescence spectroscopy, intensity fluctuations from the emitted light are measured from either single fluorophores, or pairs of fluorophores. This decay in fluorescence can be measured over a wide time range: from picoseconds to milliseconds and beyond.